BIOEXCEL M

BIOEXCEL M:

What is Fermentation?

Fermentor cultivates microorganisms, producing food, biomedicine (vaccine, antibiotics), vitamin, enzyme, and insecticides. One of the most well-known applications of micro-organisms in industry is fermentation, where carbohydrates are converted into an acid or an alcohol.
Fermentation is mostly extracellular activity and is brought with the help of enzymes released by the microorganisms. Microorganisms, such as fungi, algae, bacteria, protozoa, are increasingly important to industry, where they are used in a huge variety of processes ranging from food production to water treatment.

BIOEXCEL M Fermentor!

Fermentor can be categorized according to shape, uses, and cultivation method. Usually, fermentor with volume from 1L to 19L is characterized as Lab Scale Fermentor. BIOEXCEL M is a sensational concept of fermentation based on interactive control system. The BIOEXCEL M system is a lab scale fermenter and bioreactor designed for multipurpose fermentation and cell culture. This model is recommended for researcher, professor and supervisor for diversified experimental methods as well as providing intellectual communication with convenience. BIOEXCEL M controller offers various bioreactor functions. Hi-res color touch screen, easy to use GUI, self-process stage control on user basis and data logging in PC functions are adopted.

The BIOEXCEL M’s 7-inch wide control panel screen, sensor port and communication port are located in the front and allows side by side placement: 180mm(W), 15kg, slim design.  The BIOEXCEL M’s control system is easy to handle, and all the monitored data and control parameters are transmitted to a PC via serial communication.  Variable vessels are available for bacteria, yeast or fungi, and with optional accessories can be adapted for mammalian, animal, insect or plant cell. Vessels used for BIOEXCEL M are basically for microorganisms such as bacteria, yeast or fungi. However, This can be also used for mammalian, animal, and insect or plant cell culture with optional device like 4-gas mixer.  BIOEXCEL M is basically able to do batch, fed-batch, and continuous culture mode for microorganism fermentation. also, these three culture modes can be used for animal cell, plant cell, and microalgae, but please note that all functions which can satisfy customer’s every single demand cannot be offered. Sensors controlling of pH, DO, temperature and foam with 4 peristaltic pumps(acid, base, antifoam and feed), DO, pH, Temp, Foam, sensors are available. The graphic user interface program tracks the status of the fermentation system in real time. You can use 4 peristaltic pumps for feeding as well as external pumps.

Application of fermentation in Bio-Industry

Foods that are produced by using these methods include cheese, yoghurt, butter, beer, wine and bread. Fermentation is mostly extracellular activity and is brought with the help of enzymes released by the microorganisms. The end products or the various intermediate products (primary and secondary metabolites) of the fermentation activities of many microorganisms are highly useful. Hence, microorganisms have been commercially extended by fermentation industries. One of the most important features of fermentation is that micro-organisms can be grown in a physiological steady state. In steady state, growth occurs at a constant rate and all culture parameters remain constant (culture volume, dissolved oxygen concentration, nutrient and product concentrations, pH, cell density, etc.). In addition environmental conditions can be controlled by the experimenter. Micro-organisms grown in chemostats naturally strive to steady state: if a low amount of cells are present in the bioreactor, the cells can grow at growth rates higher than the dilution rate, as growth isn’t limited by the addition of the limiting nutrient. The limiting nutrient is a nutrient essential for growth, present in the media at a limiting concentration. Micro-organisms grown in chemostats naturally strive to steady state: if a low amount of cells are present in the bioreactor, the cells can grow at growth rates higher than the dilution rate, as growth isn’t limited by the addition of the limiting nutrient. The limiting nutrient is a nutrient essential for growth, present in the media at a limiting concentration.

The BIOEXCEL M system is a lab scale fermenter and bioreactor designed for multipurpose fermentation and cell culture. This model is recommended for researcher, professor and supervisor for diversified experimental methods as well as providing intellectual communication with convenience. Usually, fermentor with volume from 1L to 19L is characterized as Lab Scale Fermentor.
The BIOEXCEL M’s control system is easy to handle, and all the monitored data and control parameters are transmitted to a PC via serial communication. The BIOEXCEL M’s 7-inch wide control panel screen, sensor port and communication port are located in the front and allows side by side placement and slim design.
Variable vessels are available for bacteria, yeast or fungi, and with optional accessories can be adapted for mammalian, animal, insect or plant cell. Vessels used for BIOEXCEL M are basically for microorganisms such as bacteria, yeast or fungi. However, This can be also used for mammalian, animal, and insect or plant cell culture with optional device like 4-gas mixer. Additional exterior 4 channel gas mixer can be used.
Easy to do Batch, Fed-Batch, Continuous culture fermentation, and it enables to cultivate micro-organism by accurate adjustment of temperature, agitation speed, pH, DO, and foams (which generate during the fermentation) via microprocessor and PC. Also, these three culture modes can be used for animal cell, plant cell, and microalgae, but please note that all functions which can satisfy customer’s every single demand cannot be offered.
Sensors controlling of pH, DO, temperature and foam with 4 peristaltic pumps(acid, base, antifoam and feed), DO, pH, Temp, Foam, sensors are available.
BIOEXCEL M is a sensational concept of fermentation based on interactive control system. Color touch screen and easy to use GUI, self-process stage control on user basis and data logging in PC functions are adopted. Core competence of BIOEXCEL M is providing perfect control reflecting the newest IT trend and changeover ideas for users to develop their own methods.
BIOEXCEL M Controller PID controls pH, Temperature, Agitation speed, and DO by Cascade method. Cascade method is adopted for agitation speed or mixing gas. Fed-batch culture are available based on pH, DO. This fed-batch culture method maintains vessel inside condition based on constant level of pH, DO. Continuous culture can be also used as continuous feeding is available in the system.
BIOEXCEL M Controller supports pH, Do, temperature and foam sensor. It also includes 4 peristaltic pumps for Acid, Base, Antifoam, and feeding for fermentor.
Generally, motor for agitation is installed in top plate of vessel, and simple and durable agitation motor is used. In some case, Bottom magnetic Drive system can be applied. Various size options are available for Single vessel, Double wall vessel, Bowl vessel, Single round bottom vessel, and Small volume vessel. For cell culture such as animal or plant cell, servo motor can be used for agitation in very slow speed with accurate control of speed.
Digital PID control algorithm can control the temperature very fast, and stable temperature is maintained. pH value is calibrated in consideration of temperature, and can be controlled by feeding acid and base during fermentation. DO value is adjusted to measure accurate value according to temperature and salinity.

• BIOTRON BIOEXCEL M Fermentation system is a lab scale bioreactor designed for multipurpose cell culture.
• The size of vessel can be decided upon the working volumes of real media and we provide interchangeable vessels that are convenient to scale up vessel among the various size of vessels.
• Qualified for dense fermentation : Batch fermentation, DO-stat and pH-stat fed-batch, continuous culture, etc. with microprocessors controlling Temp, pH, DO, agitation and peristaltic pumping for various fermentation procedures on the control panel.
• BIOEXCEL M Control system shows the measured values of Temperature, pH, DO, Foam, Motor speed and the status of peristaltic pumps at the same time.
• Monitor and manage data from anywhere! Save and manage data on a PC by simply connecting the controller to a PC.
• The graphic user interface program tracks the status of the fermentation system in real time.
• You can use 4 peristaltic pumps for feeding as well as external pumps.
• All measured data and control parameters are transmitted to a PC and wireless communication protocol.
• The BIOEXCEL M bioreactor’s 7-inch wide control panel screen, Built-in 7” color touch screen; easy-to-learn controller with intelligent GUI.
• Sensor port and communication port are located in the front for the user’s convenience and allows side by side placement: 180mm (W), 15kg, and slim design. Side-by-side location of vessel and controller: 4 peristaltic pump and pH, DO, foam, temperature sensor port.
• Easy to calibrate temperature, pH, DO, foam sensors when using graphic calibration mode.
• The motor speed can be controlled by the DO value in DO mode to control the dissolved oxygen level. Agitation motor speed controlled by Dissolved oxygen rate in the DO mode automatically.
• User can control fed batch by DO mode or accelerate motor speed Fed batch function and motor speed controls can be controlled by DO control mode.
• User can choose feeding time so as to have stability and controllability.
• The pH and DO value precisely with the temperature, and the value of dead-band for the peristaltic pump control can be adjusted. pH, DO offset: A operating instant of pH can be setting up by user, which resolve any control problems are occurred. User can choose feeding time so as to have stability and controllability. DO value from Dead band lead to certain point of feeding.
• Intelligent self-diagnostic system to maintain fermentation process from start to end: Diagnostic, Setup, Dead Band, Offset, Warning, Feeding condition.
• Easy control of external device by fermentation controller. And PC remote controls via data logging system.
• Connected to glucose analyzer, Collect signal and send it to computer system by MFC device. (Option)
• Pure glass touchscreens for superior image clarity durable, scratch resistant glass surface Finger, gloved hand, soft stylus activation Fast sensitive touch response Flat, spherical, cylindrical touchscreen,
• Trend Data Viewer: BIOEXCEL M controller shows parameter data such as pH, DO, Temperature, Agitation, Pump status during fermentation process in real time. For PC, this data is displayed as graph and past graph is accumulated and showed on the monitor.
• Graph check and PID Value adjustment for temperature Test. By adjusting PID according to install environment, accurate temperature control is available. Temperature control test function shows actual temperature control situation on the graph.

Lab Scale Fermentor BIOEXCEL M : Fermentation Culture Method

Batch Culture

A closed system initiated by the inoculation of microorganisms under certain environmental conditions such as specific nutrients, temperature, pressure, aeration and so on. Only few generations of microorganisms can grow before given nutrients are vanished.

1. Closed system : only open to air
2. Surface culture, submerged culture
3. Parameter monitor & control
4. Biomass growing up

Fed-Batch Culture

One or more nutrients (substrates) are fed to the bioreactor during cultivation and in which the product or cultured broth remains in the bioreactor until the end of the run.

1. Open system
2. High cell density culture
3. Extended cultivation time
4. Increase cell efficiency

Continuous Culture

Cell culture method which keeps culture volume constant. Fresh medium flows into bioreactor continuously and part of the medium in the reactor is withdrawn from the bioreactor at the same flow rate of the inlet flow

1. Chemostat : constant chemical environment characteristic of the steady state
2. Control the growth rate with feeding rate
3. Strain selection: faster growth
4. Contamination : strict maintenance
5. Cell physiology research

Fresh growth medium is added continuously during fermentation and spent medium is removed. (or cells itself are removed)

Explanation Of BIOEXCEL M Fermentor Vessels

SINGLE VESSEL

• It is used for microorganism cultivation and high speed agitation
• (It is not used for animal cell fermentation as rpm speed should be slow)
• Single vessel is relatively cheaper than double vessel
• Low risk of glass damage from shock..
• Temperature: single vessel does not require outside temperature control equipment.

DOUBEL VESSEL

• Heat transfer is stable due to larger heat transfer surface area.
• Useful for quick cooling and decreasing temperature.
• Water Bath is required for temperature control.
• No congested area due to round shape bottom.

BOWL VESSEL

• Bottom part is stainless.
• Low risk of damage from shock.
• Production capacity : Maximum total volume 19L.

Characteristics of heat :

• Heat transfer surface area is larger than single vessel
• Temperature cooling is faster than single vessel
• It is a suitable combined form between single vessel and double vessel, and requires temperature controlling equipment (chiller or water bath)
• Bottom stainless part is double jacket which can control vessel inside temperature by heating or cooling, so cold finger is not required. For huge volume, bowl vessel is most appropriate choice

SMALL VOLUME VESSEL

• Small volume vessel below 1L are primarily for animal or stem cell cultivation.
• As surface area of head plate is very limited on vessel below 1L, pH, DO, Temp, Foam, ORP Sensors, and etc. cannot be equipped and used on the top of the vessel.
• In order to overcome such problem, sampling, condenser, feeding ports, and sensor ports are inserted in the side of the vessel as the form of several glass tubes with certain gradient.
• The number, as well as size and shape, of side glass tubes can be adjusted according to customer’s request.
• Customized design of the vessel can be offered according to the micro-carrier the user selected for high density cell cultivation.
• The vessel can be customized for both adherent cell and suspension cell culture. Also, spin filter or depth filter type is available.
• For stem cell cultivation, customized design is also available for assembling form of vessel with different heights of flat type or insertion of membrane inside the vessel.