BIOEXCEL MB

BIOEXCEL MB AND BIOEXCEL MF

BIOEXCEL MF can be used to conduct an experiment in same environmental condition, but with different variable. For example, under same pH, temperature and agitation speed, multi fermentation system measures the change of biomass when different DO values are applied on each vessel. Hence, its advantage is to quickly and accurately deduct a comparative data according to change in certain variable under certain condition. When only one single fermentor is used for comparative experiment, it is not only time taking and expensive to rerun at same condition, but also very erroneous to maintain surrounding environment and conditions inside vessel.

Each fermentor can independently set up pH, DO, Temperature, agitation speed, and foam sensitivity. Status for Air pump on/off, agitation, run, and remote control is recorded as graph. Setup status of DO control mode and Fed batch mode is indicated on the controller.

On PC, each fermentor can be set up with each function, and remotely control for Run and stop. To be more specific, parameters, such as pH, DO, Temperature, Agitation speed, and foam values, as well as DO stat Fed batch, pH stat fed-batch, user feeding, and Gas control can be set up.

BIOEXCEL MF is a Multi Fermentation system for microorganism culture, and BIOEXCEL MB is a Multi Bioreactor System for animal cell, plant cell, and microalgae culture.

Parallel process available for both enzymatic bioconversion and microorganism reaction. Automatic medium transfer available from a previous vessel to the next vessel after a reaction or culture.

BIOEXCEL MF Multi Fermentor is a multi-fermentation system designed to look for optimum fermentation environment or medium optimization by comparative experiment.

BIOEXCEL MB    BIOEXCEL MF

■ Vessel : 0.5L~19L

■Temp : Ambient +3℃~80 ℃

■Speed : 20RPM ~ 2000RPM

BIOEXCEL MB system is based on BIOEXCEL A Bioreactor. BIOEXCEL MF system is based on BIOEXCEL M Fermentor. They can be connected to a maximum of 6 bioreactors so that it forms a unified multi- fermenter/bioreactor system.

Fermentation, pH Stat Fermentation, Gas analysis formula calculation (RQ, CER, OUR), Growing environment graph indicated. pH control through Gas influx regulation (CO2, NH3), Alarm function. Temperature PID Control, culture medium, mixed gas influx, Analog data input Feed control.

• Multi-vessel : 2-6 channels
• Total volume 250 ml ~ 19L per reactor
• Independent control and dependent control
• Convenient, reliable controller system
• Minimization footprint with the compact design
• pH, DO, Temp, foam, agitation, pump, air flow etc.
• Scale-up is available with the same controller
• Spargers for submerse gassing
• Condenser for drying of the off-gas
• Easy to see inside of the glass vessel
• High density cell culture available
• Easy to setup feeding program
• Graphic pH & DO calibration

OPTION

BIOEXCEL MF Fermentation system basically consists of 2, 4 or maximum 6 fermentor vessels as one fermentation system. It is designed to be used as either a stand-alone or a multiple bioreactor system. 3, or 5 fermentor vessels can be also offered according to customer’s demand. Through PC program, maximum 6 fermentor vessels can be used for comparative experiment simultaneously. Furthermore, all data during fermentation process can be collected, that data or graph from all channels can be simultaneously saved. Several main functions can be controlled remotely.

• MFC, Gas Analyzer, Auto Sampler
• Mass Flow Control – O2, CO2, N2, AIR
• Analysis of O2 , CO2
• Software Indication of OTR, CTR

All products are designed to be arranged frontally : Display, Air flow meter and Gas Mixer, 4 Peristaltic pumps, Gas outs, Feeding ports, Temp/Agitation/Heating and pH/DO/Foam/Temperature Sensor cable connection, etc.

BIOEXCEL MB or BIOEXCEL MF Multi bioreactor system are able to communicate via personal computers, RS-232 or wireless connections. By using a personal computer, you can easily and conveniently conduct integrated management and compare each bioreactor and bioreactor process. You can also save and control data interactively.

Multi Fermentor BIOEXCEL MF

BIOEXCEL MF Multi Fermentation system can be categorized according to shape, uses, and cultivation method. Usually, fermentor with volume from 1L to 19L is characterized as Lab Scale Fermentor. The BIOEXCEL MF system designed for multipurpose fermentation. BIOEXCEL MF is basically able to do batch, fed-batch, and continuous culture mode for microorganism fermentation. Vessels used for BIOEXCEL MF are basically for microorganisms such as bacteria, yeast or fungi.

Summary Of Features And Strength

• Maximum 6 bioreactor and fermentor vessels for comparative experiment
• Easy operation on touch screen controller with color LCD monitor and easy data check with graph
• Controller prevents arbitrary change in cultivation condition by setting user password.
• Reduction in study period, labor force and cost through simultaneous experiment.
• Comparative experiment for different variables under same condition and for parameters.
• Each can be controlled independently and concurrently.
• Various types and size of vessel can be replaced: Single, Double, Bowl, Single round and small volume vessel and spin filter for perfusion culture and depth filter vessel for animal cell culture
• Continuous culture available: Reaction and cultivation in Number 1 vessel. Transfer to number 2 vessel automatically, and then reaction and cultivation in number 2 vessel.
• Enzymatic Bioconversion and microorganism reaction simultaneously
• all data during fermentation process can be collected, that data or graph from all channels can be simultaneously saved
• On PC, each fermentor can be set up with each function, and remotely control for Run and stop.
• Automatic control of DO value by motor speed change
• Compact System : Minimization of working space for multi process
• Remote control by PC software, data logging system.
• Excel file restore available of real-time data an graph
• Scale-up is available with the same controller
• Built in peristaltic pumps for anti-foam, feeding and pH control, fed batch, continuous culture.
• Multi-fermentor suitable for various experiments simultaneously.
• Individual experiment can be performed by each fermentor.
• Experiment at same conditions and different parameters
• Experiment at different conditions with same organisms
• Compatible with various types and dimensions of vessels
• Shortens study period and saves human resource and cost
• Sensor port and communication port on front side for user’s convenience.
• Secures the cell culture process with password protection
• Accurate PID temperature control system.
• Reliable operation due to automatic controllers.
• Remote control available in PC with data logging system.
• Save and manage data on PC by connecting controller to PC.
• Self-data logging system within fermentation controller.
• Intelligent GUI software with 7” wide screen and easy operation with touch panel.

• BIOEXCEL Multi is for multiple culturing experiment and able to compare the results simultaneously.
• Maximum 6 vessels and controller can be arranged in one case in a row or independently arranged in a row.
• Large volume up to 84L (total volume) with space saving design and user basis controller program
• This model is recommended to use in laboratory, industrial R&D center, bioprocess institutes and biomass Company.
• BIOEXCEL PM consists of BIOEXCEL M controllers in 2channel / 4channel / 6channel, and is made into slimmer shape to be convenient for user through plate work.
• Various type of vessels and peristaltic pumps for scale-up and accurate operation.
• Parallel design of vessel and controller: 4 peristaltic pump and pH, DO, foam, temperature sensor port.
• Built in peristaltic pumps for anti-foam, feeding and pH control, fed batch, continuous culture.
• Programmable multi stage operation: Setting pH, DO, Foam, temperature for each stage to the end of the fermentation process.
• Easy to calibrate temperature, pH, DO, foam sensors in use of graphic calibration mode.
• Qualified for dense fermentation: DO-stat, pH-stat, Exponential feeding, etc.
• Intelligent self-diagnostic system to maintain fermentation process from start to the end: Diagnostic, Setup, Dead Band, Offset, Warning, Feeding condition.
• BIOTRON BIOEXCEL PM offers not only wireless network observation and control system but central controlling when even each controllers remain away.
• Hi-res color touch screen and easy to use GUI, self-process stage control on user basis and data logging in PC functions are adopted.
• Core competence of BIOEXCEL M is providing perfect control reflecting the newest IT trend and changeover ideas for users to develop their own methods.
• Easy control of external device by fermentation controller: O₂/CO₂ analyzer, glucose analyzer, spectrophotometer, fluorescence, spectrophotometer, NIR, gas mixer, level, weight, etc.

SINGLE VESSEL

Top Plate (pH, DO, anti-foam, Thermo well, Inoculation, Sampling 4point feeding ports) Ports Cap, Blade, Foam Breaker, Pyrex Glass condenser, Ring type Sparger, Removal Baffle Powerful DC Motor, Single Mechanical Seal, Pyrex Glass Vessel silicon Tube, Vessel Packing, cold Finger Heating Plate (Heater, Water in/out ports)

• Type : Single Wall and Flat Bottom
• Vessel material: Borosilicate glass
• Top Plate  : SUS 316L
• pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Cold Finger, Sparger, Heater Plate
• Air Condenser : SUS Condenser (Stainless Steel)
• Vessel glass packing and Vessel port packing
• Inlet filter : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
• Outlet filter :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
• Flow control : Manual
• Sampling port : T-pipe type manual sampler

DOUBLE VESSEL

Pyrex Glass double Vessel Assembly with Vessel supporter Top Plate (pH,DO,Anti-foam, Thermo well, Inoculation, Sampling 4poin feeding points). Ports, Blade, Foam Breaker Pyrex Glass Condenser, Ring type Sparger, Removal Baffle Powerful DC Motor, Seal, Pyrex Glass Vessel Silicon Tube, Vessel packing, Cold Finger Heating Plate(Heater, water in/out ports) * Double vessel doesn’t need Heating Plate but Water bath for exclusive use

• Type               : Double Wall and Dished Bottom type
• Vessel material  : Borosilicate glass
• Top Plate  : SUS 316L
• pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Sparger
• Air Condenser   : SUS Condenser (Stainless Steel)
• Vessel glass packing
• Vessel port packing
• Inlet filter           : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
• Outlet filter         :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
• Flow control         : Manual
• Sampling port       : T-pipe type manual sample

BOWL VESSEL

Pyrex glass single Vessel with BOWL type low plate Top Plate (pH, DO, Anti-foam, Thermo well, Inoculation, Sampling 4 point feeding ports SUS ports Cap, Blade, Foam Breaker Pyrex Glass Condenser, Ring type sparger, Removal Baffle Powerful DC Motor, Single Mechanical Seal, Pyrex Glass Vessel Silicon Tube, Quick coupling, Vessel Packing Heating Plate (Heater, Water in/out ports)

• Type               : Single Wall and Bowl Bottom
• Vessel material  : Single well – Borosilicate glass, Bowl – SUS316L
• Top Plate  : SUS 316L
• pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Sparger
• Air Condenser   : SUS Condenser (Stainless Steel)
• Vessel glass packing
• Vessel port packing
• Inlet filter           : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
• Outlet filter         :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
• Flow control         : Manual
• Sampling port       : T-pipe type manual sampler

SMALL VOLUME VESSEL

SINGLE ROUND VESSEL

Mass cultivation of animal cells has intensively been investigated even thought cell culture techniques have been developed for long times. There have been several difficulties in maintaining large quantities of live cells, especially more than million of cells per m/ in over five liter vessel. Fragility of animal cell, sophisticated culture conditions less economical feasibility of being scaled-up were major bottle necks in idustrializing cell culture technologies to produce a large amount of pharmaceuticals, tPA, interferon, EPO and many other MAb.

• Type : Single round Vessel
• Vessel material: Borosilicate glass
• Top Plate  : SUS 316L
• pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Cold Finger, Sparger, Heater Plate
• Air Condenser : SUS Condenser (Stainless Steel)
• Vessel glass packing and Vessel port packing
• Inlet filter : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
• Outlet filter :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
• Flow control : Manual
• Sampling port : T-pipe type manual sampler